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Actin monomers are attached by TB-500, which also sequesters G-actin and promotes F-actin assembly

However, the simultaneous and selective determination of both forms (GSH and GSSG) was a great challenge

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Cysteine can be synthesized from methionine or through a salvage pathway, but the knockout mice are not able to maintain an adequate supply of cysteine for growth and development while continuously excreting large amounts of GSH in the urine